Keep my session open?
Ending In 
Your shopping session has expired.
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

Anti-HGF Goat Polyclonal Antibody

Supplier: Genetex
GTX10680
Goat polyclonal to HGF

For ELISA: Use at a concentration of 0.5 - 1.0 μg/ml, this detects 0.06 ng/well of rhHGF. For IHC-P: Use 15 μg/ml using human samples. For WB: Use at a concentration of 0.1 - 0.2 μg/ml, this detects rhHGF at 25 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 82 kDa. For Neut: Use at a concentration of 0.2 - 0.6 μg/ml. Anti-Human HGF is tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF is preincubated with various dilutions of the antibody for 1 hour at 37 °C, then placed in a 96-well microtiter plate. 4MBr-5 cells are added to each well and incubated for 48 hours at 37 °C in a 5% CO2 humidified incubator and then pulsed for the last 24 hours with ^3H-thymidine. Cells are harvested onto glass filters and the ^3H-thymidine incorporation into DNA is measured. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Type: Primary
Antigen: HGF
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype: IgG
Reactivity: Human




 

ORDER

Expand All / Collapse All
Size Supplier No. VWR Catalog Number Unit Price Quantity
25μg GTX10680 89354-790 Each (25µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

Generate Barcodes Barcode Label Format: 
Generate PDF Catalog Page

SPECIFICATIONS

Antigen Symbol HGF
Antigen Name Hepatocyte growth factor
Reactivity Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody Type Primary
Host Goat
Isotype IgG
Western Blot Yes
ELISA Yes
ImmunoChemistry Yes
Neutralization Yes
Antigen Synonyms SF, HGFB, Hepatocyte growth factor, F-TCF, Lung fibroblast-derived mitogen, DFNB39, HPTA, Hepatopoeitin-A, Fibroblast-derived tumor cytotoxic factor
Gene ID 3082
Concentration 0.1mg/ml
Immunogen Recombinant, human HGF (rhHGF) expressed in SF 21 as immunogen.
Purification Immunogen affinity purified
Storage Buffer Phosphate-buffered saline, pH 7.4, containing 5% trehalose
Storage Temperature Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Tested Applications ELISA, IHC-P, Neut, WB
Enter Details

Select the article for which you are looking for Certificate

Indicate the Lot Number. This field is mandatory.

Enter Certificate Details

Enter the Lot number:

Choose from recent batch/lot numbers:

Or
Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty
 

A new look and a renewed commitment

Built on the features & resources you know, optimized with innovation from Avantor®. Our new brand symbolizes agility and our ability to adapt to customer needs.

Learn More Start Shopping