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Anti-HGF Mouse Monoclonal Antibody [clone: 24612.111]

Supplier: Genetex
GTX10678
Mouse monoclonal [24612.111] to HGF

ELISA: Use at a concentration of 0.5 - 1 μg/ml to detect <1.2 ng/well of rhHGF. Neut: Use at a concentration of 0.01 - 0.5 μg/ml. WB: Use at a concentration of 1 - 2 μg/ml to detect rhHGF at 50 ng/lane under non-reducing conditions. Predicted molecular weight: 83.1 kDa. Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the antibody for 1 hour at 22°C, then placed in a 96-well microtiter plate. 4MBr-5 cells were added to each well and incubated for 48 hours at 37°C in a 5% CO2 humidified incubator and then pulsed for the last 24 hours with ^3H-thymidine. Cells were harvested onto glass filters and the ^3H-thymidine incorporation into DNA was measured. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: HGF
Clonality: Monoclonal
Clone: 24612.111
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg GTX10678 89354-780 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Antigen Symbol HGF
Antigen Name Hepatocyte growth factor
Reactivity Human
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone 24612.111
Host Mouse
Isotype IgG1
Western Blot Yes
ELISA Yes
Neutralization Yes
Antigen Synonyms SF, F-TCF, Hepatocyte growth factor, Fibroblast-derived tumor cytotoxic factor, Lung fibroblast-derived mitogen, Hepatopoeitin-A, DFNB39, HPTA, HGFB
Gene ID 3082
Concentration 0.5mg/ml
Immunogen Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21.
Purification Protein A purified
Storage Buffer PBS containing 5% Trehalose
Storage Temperature Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Tested Applications ELISA, Neut, WB
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