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Anti-CXCR4 Mouse Monoclonal Antibody [clone: 44716.111]

Supplier: Genetex
GTX10403
Mouse monoclonal [44716.111] to CXCR4

FACS: Use 10 μg/ ml is for 10 μl (10 ^5-6 ) cells. IF: Use at a concentration of 5 μg/ml (determined using stimulated human peripheral blood mononuclear cells (PBMC) fixed with 2% formaldehyde and permeabilized with 0.1% saponin). IHC: Use 0.5 -5 μg/ ml (determined using human CXCR4 cultured cells or tissue sections.) Inhib: For neutralization, a working concentration of 2.5-20 μg/ml of CXCR4 will block 50% of the bioactivity due to 0.1 μg/ml recombinant human SDF1 in an assay measuring chemotaxis using BaF/ 3 human CXCR4 transfected cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of the cell surface CXCR4 mediated SDF1/ PBSF response on a responsive cell line. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Type: Primary
Antigen: CXCR4
Clonality: Monoclonal
Clone: 44716.111
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity:




 

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 μg GTX10403 89350-202 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Antigen Symbol CXCR4
Antigen Name Chemokine (C-X-C Motif) Receptor 4
Reactivity Cat
Conjugation Unconjugated
Clonality Monoclonal
Antibody Type Primary
Clone 44716.111
Host Mouse
Isotype IgG2b
ImmunoChemistry Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen Synonyms LCR1, WHIMS, WHIM, D2S201E, LAP3, NPY3R, HM89, NPYRL, LAP-3, FB22, NPYR, NPYY3R, CD184, LESTR, HSY3RR
Gene ID 7852
Concentration 0.5mg/ml
Immunogen Human CXCR4 transfected NSO mouse myeloma cells.
Purification Protein G purified
Storage Buffer Preservative: NoneConstituents: PBS Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.
Storage Temperature Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Tested Applications Fluorescence-Activated Cell Sorting, Inhibition
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