3-Amino-9-ethyl carbazole (AEC) Chromogen/Substrate
Supplier: Novus Biologicals
Synonyms:
9-Ethylcarbazol-3-ylamine
, 9-Ethyl-9H-carbazol-3-amine
, AEC
Even though DAB is much more sensitive chromogen compared to amino ethyl carbazol (AEC) because of its carcinogenic nature, some labs avoid using DAB. To resolve this problem, we have designed an AEC chromogen in liquid format. AEC is a chromogen of choice when performing immunoperoxidase staining. It has been well accepted amongst the pathologist because of its relatively less toxic nature compared to DAP. It produces a red colored end product at the positive sites which gives a good contrast with blue hematoxylin counter stain. Specimens stained in AEC cannot be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium.
Specificity: Peroxidase reacts with 3% Hydrogen Peroxide Substrate to degrade it, which in turn reacts with AEC to precipitate it at the positive sites giving red brown colored end product.
Caution: This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Formula:
C₁₄H₁₄N₂ MW: 210.28 g/mol |
MDL Number:
MFCD00004964 CAS Number: 132-32-1 |
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