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E.Z.N.A.®Plasmid Mini Extraction Kits, Omega Bio-tek

Supplier: OMEGA BIO-TEK
Omega Bio-tek provides various methods for plasmid DNA extraction from bacterial cultures. The E.Z.N.A.® Plasmid Mini systems use the alkaline-lysis method to generate bacterial lysates combined with silica membrane technology to purify plasmid DNA. The Plasmid Mini Kit I is our most popular kit that yields up to 30 µg plasmid DNA from 1 – 5 mL bacterial culture. Plasmid DNA is bound to a HiBind® DNA column membrane, purified with a few wash steps, and eluted in a low-salt buffer. The Plasmid Mini II system uses this same purification method as the Plasmid Mini Kit I, isolating plasmid from up to 15 mL bacterial culture. Purified plasmid is ready for downstream applications such as sequencing, restriction digestion, transfection, and transformation.

The E.Z.N.A.® Endo-Free Plasmid DNA systems are designed to efficiently purify plasmid DNA with low endotoxin levels for Transfection. . The Endo-Free Mini Kit I isolates plasmid from 1 – 5 mL bacterial cultures. After cell lysis and neutralization, the lysate is cleared via centrifugation and treated with a reagent for endotoxin removal. Plasmid DNA is purified with a few wash steps and eluted, yielding plasmid DNA that is ready for use in sensitive cell line transfection and in vitro experiments. Transfection-grade plasmid purification is also achieved with the Endo-Free Plasmid Kit II from up to 15 mL bacterial culture.

The BAC/PAC DNA Kit uses a alkaline lysis buffer system to separate genomic DNA from BAC and Cosmid DNA. The X-press Plasmid DNA Kit can isolate plasmid DNA in less than 7 minutes by eliminating the clearing of cellular debris. The DNA isolated with this system can be used for sequencing and restriction enzyme digests.

Plasmid isolation systems are also available in Midi and Maxi, magnetic bead, and high throughput formats.




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Description Format Binding Capacity Culture Volume Sample Type Kit Size Supplier No. VWR Catalog Number Unit Price Quantity
E.Z.N.A. BAC/PAC DNA Kit - 35 μg 1.5 - 5 mL 5 Preps Bacteria D2156-00 101414-376 Each Retrieving Restricted
Minority or Woman-Owned Business Enterprise
E.Z.N.A. Plasmid Mini Kit II Mini Spin 70 μg 5 - 10 mL 5 Preps - D6945-00 101318-856 Each Retrieving Restricted
Minority or Woman-Owned Business Enterprise
E.Z.N.A. Plasmid Mini Kit II Mini Spin 70 μg 5 - 10 mL 200 Preps - D6945-02 101318-860 Each Retrieving Restricted
Minority or Woman-Owned Business Enterprise
E.Z.N.A. Plasmid Mini Kit II Mini Spin 70 μg 5 - 10 mL 50 Preps - D6945-01 101319-320 Each Retrieving Restricted
Minority or Woman-Owned Business Enterprise
E.Z.N.A. X-Press Plasmid Mini Kit Mini 20 μg 1.5 mL 50 Preps - D6947-01 95043-086 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Minority or Woman-Owned Business Enterprise
restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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ACCESSORIES

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Accessories
Description Size Kit Size Supplier No. VWR Catalog Number Unit Price Quantity
Neutralization Buffer for Silica Spin Column Methods, Solution 3 250 mL - PS003 95043-188 Each Retrieving Restricted
Lysis Buffer for Plasmid DNA Kits, Solution 2 250 mL - PS002 101414-810 Each (250ml) Retrieving Restricted
Resuspension Buffer, Solution 1 250 mL - PS001 95043-186 Each Retrieving Restricted
Elution Buffer - 100 mL PDR048 101414-778 Each (100ml) Retrieving Restricted
HiBind DNA columns - 200 DNACOL-02 95043-212 Each Retrieving Restricted
HiBind DNA columns - 50 DNACOL-01 95043-210 Each Retrieving Restricted
IPTG, Solid Powder, >99% 5 g - AC121 101414-324 Each (5g) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

RNase A 400 µL - AC117 101414-312 Each (400µl) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SPM Wash Buffer Concentrate, 40 mL - - PS014 95043-204 Each Retrieving Restricted
X-Gal, Solid Powder, >99% 1 g - AC120 101414-314 Each (1g) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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