Keep my session open?
Ending In 
Your shopping session has expired.
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

Bst DNA Polymerase, Lucigen

Supplier: LUCIGEN CORPORATION
30027-1 30027-2 30028-1
Lucigen's Bst DNA Polymerase, Exonuclease Minus, has the highest strand displacement activity available

It is used in isothermal amplification and Next Generation Sequencing. Applications include Strand displacement amplification, DNA sequencing through high GC regions, and Rapid sequencing from nanogram amounts of DNA template.

Bst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5'-3' polymerase activity and strand displacement activity, but it lacks 3'-5' exonuclease activity. It also has reverse transcription activity.

Lucigen's Bst DNA Polymerase, Exonuclease Minus, has higher strand displacement activity than that of other suppliers. The enzyme can be used in nucleic acid amplification methods such as isothermal amplification, whole genome amplification (WGA), and multiple displacement amplification (MDA). It also can be used in Next Generation sequencing.

This enzyme has optimal activity at 65°C. It is suitable for sequencing DNA with high GC content and secondary structures. It is available in concentrations of 8,000 U/ml or 50,000 U/ml. Bst DNA Polymerase, Exonuclease Minus, is supplied with 10X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100.

Tips for use: 1) Requires 0.1% Triton X-100 for long term storage. 2) Reaction temperatures above 70°C are not recommended. 3) Bst DNA Polymerase cannot be used for thermal cycle sequencing. 4) Heat Inactivation: 80ºC for 20 min.

Ordering information: Bst DNA Polymerase, Exonuclease Minus is available in two concentrations, 8,000 U/ml and 50,000 U/ml, in a storage buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol. Also supplied is 10 X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100. Note: Some uses for this product may require licenses. Lucigen does not encourage or support the unauthorized or unlicensed use of patented nucleic acid amplification processes for isothermal amplification, whole genome amplification (WGA), multiple displacement amplification (MDA), and Next Generation sequencing. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties. If the purchaser is not willing to accept these use limitations, Lucigen Corporation is willing to accept return of the product for a full refund.




Log in to see your contract pricing and availability.
  Remember me on this device
 

ORDER

Expand All / Collapse All
Description Size Units Supplier No. VWR Catalog Number Unit Price Quantity
Bst DNA Polymerase, Exonuclease Minus 50,000 U/mL - 10000 30028-1 95059-676 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Bst DNA Polymerase, Exonuclease Minus 8,000 U/mL - 2000 30027-1 95059-672 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Bst DNA Polymerase, Exonuclease Minus 8,000 U/mL 5 x 2,000 units 10000 30027-2 95059-674 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

Generate Barcodes Barcode Label Format: 
Generate PDF Catalog Page

Enter Details

Select the article for which you are looking for Certificate

Indicate the Lot Number. This field is mandatory.

Enter Certificate Details

Enter the Lot number:

Choose from recent batch/lot numbers:

Or
Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty