CytoScint™ ES is a complete, ready-to-use environmentally safe liquid scintillation solution designed for “one-step” counting of filters, Thin-Layer Chromatography scrapings, and samples solubilized in Hyamine® Hydroxide.
- Environmentally Safe
- Biodegradable
- One Step Filter Counting
- Non-Toxic and Non-Flammable
- Stable Counting Performance
- No Waiting on Chemiluminescence
- High Quench Resistance
For most samples containing minimal amounts of water (0-15%), CytoScint™ ES will result in increased count recovery and maximum sample solubilization. It features a high tritium performance.
It works in a special way to allow most samples collected on solid supports to be counted in one, easy step, without the necessity of pretreatment. The presence of small amounts of water will actually enhance the solubilization of most samples in CytoScint™ ES. Wet filters and thin-layer chromatography scrapings should be placed
in a vial containing 5-10 mL of CytoScint™ ES. Shake or allow the solution to stand until it clears. If the solution remains unclear after a few minutes, increase the amount of cocktail. Generally, 10 mL of CytoScint™ ES will solubilize up to 15% of aqueous sample resulting in a homogeneous counting solution.
Tissue solubilizers such as Hyamine Hydroxide® can be counted in CytoScint™ ES, which is specially formulated to resist chemiluminescence. After digesting the sample, cool and de-color (if necessary). Then add 10-15 mL of cocktail, shake until thoroughly mixed and let the solution stand for 30 minutes before counting. Precipitates (i.e. double antibody complex) may be solubilized in dilute NaOH or HCI and then added to CytoScint™ ES for high counting efficiency.
Special Characteristics - CytoScint™ ES is a safe, virtually odorfree cocktail which eliminates the need for special handling or storage. CytoScint™ ES can be used with polyethylene vials without the loss of sample due to diffusion, often a problem with conventional cocktails.
