BCIP/NBT substrate (1-Component)

Supplier: KPL

Synonyms: NBT (nitro-blue tetrazolium chloride)BCIP (5-bromo-4-chloro-3-indolylphosphate toluidine salt)

5420-0033 5420-0038 5420-0036
95059-178EA 588.26 USD
95059-178 95059-184 95059-180
BCIP/NBT substrate (1-Component)
BCIP/NBT

BCIP/NBT Membrane Phosphatase Substrate is ideal for immunoblotting and dot blot procedures


It is supplied as a convenient, one-component solution ready for use. It is compatible with film or chemiluminescent imager for flexibility in recording permanent results.


The system is based on the hydrolysis of 5-bromo,4-chloro,3-indolylphosphate BCIP/nitrobluetetrazolium (NBT) substrate by alkaline phosphatase, resulting in a strong purple reaction product with minimal background staining. It provides sensitivity in the low nanogram range, sharp band resolution and a clear image. The color produced is linear over a wide dynamic range and resists fading when exposed to light. It is supplied as a convenient, one-component solution ready for use

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