IBI provides phenol chloroform solution at a pH of 6.7 or 8.0 ± 0.2 for use in typical DNA purification
- A330: ≤0.2
- A405: ≤0.1
- A510: ≤0.1
- Accelerated shelf life assay: PASS
- Extraction assay: PASS
- Ligation/recut assay: PASS
- pH (with buffer added): 7.8-8.2
- pH range: 6.5-6.9
Store at +4°C and keep tightly sealed. IBI's phenol is prepared from ultra-pure phenol double distilled under a dry inert gas. This material is then saturated completely and extra buffer is added as an overlay to maintain saturation. This product is packaged under nitrogen for maximum stability.
To purify 400 ml of a sample, add 400 ml of phenol chloroform solution, mix well, and centrifuge for three (3) minutes. Carefully remove the aqueous layer and place into a new tube. Repeat the previous until the interface is no longer visible.
A 26 ml bottle of Tris buffer is included to adjust the pH.