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Fluorescent Calcium Indicators and Related Products

Supplier: Biotium
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Calcium is an important intracellular messenger ion responsible for the activation and deactivation of numerous biological events in cells

Calcium indicators differ in their Ca2+ dissociation constants (Kd) or Ca2+ response range, excitation/emission wavelengths, spectral shift, and relative fluorescent quantum yields. Therefore, you should select a Ca2+ indicator that best suits your need in consideration of your biological system, instrument settings and any other fluorescent probes that you may use at the same time. Kd values provide an estimate of the detectable Ca2+ concentration range, usually 0.1 Kd to 10 Kd. However, Kd values in cells could differ significantly from in vitro determined Kd values due to differences in ionic strength, pH, viscosity and Ca2+ buffering by intracellular lipids and proteins.

Biotium offers a number of Ca2+ indicators in both membrane-impermeant salt forms and membrane-permeant AM ester forms. The salt forms of the indicators are water-soluble and can be loaded into cells via microinjection. The AM esters of the indicators are membrane-permeant and thus can be loaded into cells by incubation of the cell or tissue preparation in a buffer containing the AM ester. The AM esters themselves do not bind Ca2+. However, once they have entered the cells, they are hydrolyzed by intracellular esterases into the parent Ca2+ indicators, thus becoming responsive to Ca2+. Biotium also offers related products for calcium studies, including Pluronic F-127, a mild nonionic detergent that can facilitate the loading of AM ester compounds, calcium chelators and calcium ionophores. The calcium calibration kit is used to generate buffers with a range of accurate calcium concentrations for the calibration of fluorescent calcium indicator dyes.

To study the role of calcium in these events, it is essential for one to be able to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is by the use of fluorescent Ca2+ indicators, a technique pioneered by professor Roger Tsien and colleagues (Tsien, R. in Methods in Cell Biology, Vol. 30, Taylor, D.L. and Wang, Y-L, Eds., Academic Press (1989) pp. 127-156).




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Calcium Indicators
Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Bis-Fura-2, hexapotassium salt 1 mg 50045 89139-234 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fluo-3, AM ester 10 x 100 μg 50013 89139-180 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fluo-3, AM ester 1 mg 50014 89139-182 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fluo-3, AM ester 20 x 50 μg 50016 89139-186 Each (50µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fluo-3, AM ester, 1 mM in anhydrous DMSO 1 mL 50015 89139-184 Each (1ml) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fluo-3, pentaammonium salt 1 mg 50010 89139-174 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fluo-3, pentapotassium salt 1 mg 50011 89139-176 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fluo-3, pentasodium salt 1 mg 50012 89139-178 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, AM ester 10 x 100 μg 50033-1 89139-208 Each (50µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, AM ester 20 x 50 μg 50033 89139-206 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, AM ester 1 mg 50034 89139-210 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, AM ester, 1 mM in anhydrous DMSO 1 mL 50029 89139-198 Each (1ml) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, pentaammonium salt - 50025 10018-200 Each (50µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, pentaammonium salt 1 mg 50030 89139-200 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, pentapotassium salt 1 mg 50031 89139-202 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, pentapotassium salt - 50026 10018-202 Each (500µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Fura-2, pentasodium salt 1 mg 50032 89139-204 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Furaptra (Mag-Fura-2), AM ester 20 x 50 μg 50039 89139-220 Each (50µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Furaptra (Mag-Fura-2), AM ester 10 x 100 μg 50037 89139-216 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Furaptra (Mag-Fura-2), AM ester 1 mg 50038 89139-218 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Furaptra (Mag-Fura-2), tetrapotassium salt 1 mg 50035 89139-212 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Furaptra (Mag-Fura-2), tetrasodium salt 1 mg 50036 89139-214 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Indo-1, AM ester 10 x 100 μg 50043 89139-228 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Indo-1, AM ester 20 x 50 μg 50043-1 89139-230 Each (50µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Indo-1, AM ester 1 mg 50044 89139-232 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Indo-1, pentaammonium salt 1 mg 50040 89139-222 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Indo-1, pentapotassium salt 1 mg 50041 89139-224 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Indo-1, pentasodium salt 1 mg 50042 89139-226 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Rhod-2, AM ester 10 x 100 μg 50023 89139-194 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Rhod-2, AM ester 1 mg 50024 89139-196 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Rhod-2, triammonium salt 1 mg 50020 89139-188 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Rhod-2, tripotassium salt 1 mg 50021 89139-190 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Rhod-2, trisodium salt 1 mg 50022 89139-192 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.


Ion Chelators
Description Size Supplier No. VWR Catalog Number Unit Price Quantity
5,5-Dibromo BAPTA, tetrapotassium salt 100 mg 50004 89139-162 Each (100mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

5,5-Difluoro BAPTA, AM ester 25 mg 50005 89139-164 Each (25mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

5,5-Difluoro BAPTA, tetrapotassium salt 100 mg 50006 89139-166 Each (100mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

5,5-Dimethyl BAPTA, AM ester 25 mg 50007 89139-168 Each (25mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

5,5-Dimethyl BAPTA, tetrapotassium salt 100 mg 50008 89139-170 Each (100mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

5-Methyl-5'-nitro BAPTA, tetrapotassium salt 10 mg 50009 89139-172 Each (10mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

BAPTA, AM ester 25 mg 50000 89139-152 Each (25mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

BAPTA, AM ester 20 X 1 mg 50000-1 89139-154 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

BAPTA, tetracesium salt 1 g 50001 89139-156 Each (1g) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

BAPTA, tetrapotassium salt 1 g 50002 89139-158 Each (1g) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

BAPTA, tetrasodium salt 1 g 50003 89139-160 Each (1g) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Tpen 100 mg 59003 89139-286 Each (100mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.


Ionophores
Description Size Supplier No. VWR Catalog Number Unit Price Quantity
4-Bromo A-23187, free acid 1 mg 59006 89139-292 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

A-23187, free acid 1 mg 59001 89139-282 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Ionomycin 1 mg 59007 89139-294 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.


Related Products
Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Calcium Calibration Kit 8 assays 59100 89139-296 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

DMNP EDTA (caged calcium), tetrapotassium salt 10 mg 50050 10018-204 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

EDC (EDAC) 100 mg 59002 89139-284 Each (100mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Pluronic F-127, 10% in water 30 mL 59005 89139-290 Each (30ml) Retrieving Restricted
Pluronic F-127 20% in DMSO 1 mL 59004 89139-288 Each (1ml) Retrieving Restricted
Probenecid, sodium salt, water soluble 10 mg 50027 89410-769 Pack of 10 (77mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

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  • State issued document with your organization's Federal Tax ID Number
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  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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