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Fluorescent Nerve Terminal Probes and Staining Kits

Supplier: Biotium
70039 70038 70019 70035 70034 70037 70036 70031 70030 70032 70051 70050 70028 70027 70031-1 70029 70024 70023 70045 70026 70025 70042 70020 70041 70022 70044 70021 70043 70040
Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity

Styryl dyes stain synaptic vesicles in an activity-dependent fashion. The dyes are virtually non-fluorescent in aqueous solution, but become brightly fluorescent upon insertion into the plasma membrane. During endocytosis following neuronal stimulation, the dyes become trapped in vesicles. After free dye is removed from the cell surface by washing, the remaining fluorescence is proportional to the number of newly formed endocytic vesicles. During exocytosis, the dyes are released to the aqueous medium, causing a decrease in fluorescent signal. As a result, the change in fluorescence intensity reflects the amount of endocytosis and exocytosis occuring during synaptic activity. The rate of fluorescence increase during endocytosis (on-rate) and the rate of fluorescence decrease during exocytosis (off-rate) varies from dye to dye. In general, dyes with longer lipophilic tails and more double bonds have a faster on-rate and slower off-rate. AM dyes have an additional amine group and therefore tend to be more water soluble than their FM dye counterparts, resulting in a slower on-rate and faster off-rate.

A frequent problem encountered with nerve terminal dyes is background fluorescence due to non-vesicle membrane staining. While most background fluorescence can be removed with repeated washes, background can be a problem with more lipophilic dyes, particularly in tissue preparations. Biotium offers three agents to reduce background fluorescence of nerve terminal dyes. ADVASEP-7 is a sulfonated beta-cyclodextrin that facilites removal of dye from the cell surface during washing. Biotium's unique quencher SCAS reduces background fluorescence without additional washes. Sulforhodamine 101 has been used to quench SynaptoGreen background by fluorescence resonance energy transfer (FRET). These background reducing agents are available separately or together with commonly used styryl dyes in nerve terminal staining kits.

These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (designated m) and the number of double bonds linking the two aromatic rings in the dye (designated n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

Ordering information: Sulforhodamine 101 is also available separately (catalog number 89139-504).




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Description Size Features Supplier No. VWR Catalog Number Unit Price Quantity
ADVASEP-7 250 mg Free dye clearing agent 70029 89139-416 Each (250mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

AM1-43 1 mg Ex/Em ~480/600 nm, m=3, n=1 70024 89139-406 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

AM1-44 1 mg Ex/Em ~480/600 nm, m=4, n=1 70038 89139-434 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

AM2-10 1 mg Ex/Em ~480/600 nm, m=1, n=1 70036 89139-430 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

AM3-25 1 mg Ex/Em ~480/600 nm, m=17, n=1 70051 89139-460 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

AM4-64 1 mg Ex/Em ~510/750 nm, m=1, n=3 70025 89139-408 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

AM4-65 1 mg Ex/Em ~510/750 nm, m=3, n=3 70039 89139-436 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

AM4-66 1 mg Ex/Em ~510/750 nm, m=4, n=3 70050 89139-458 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Nerve Terminal Staining Kit I 1 set 5x1 mg SynaptoGreen C4 and 250 mg ADVASEP-7 70030 89139-418 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Nerve Terminal Staining Kit IIA 1 set 1 mg AM1-43 and 100 mg ADVASEP-7 70031 89139-420 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Nerve Terminal Staining Kit IIB 1 set 1 mg AM1-43 and 100 mg SCAS 70031-1 89139-422 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Nerve Terminal Staining Kit III 1 set 5x1 mg SynaptoGreen C4 and 100 mg sulforhodamine 101 70032 89139-424 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Nerve Terminal Staining Kit V 1 set 5x1 mg SynaptoRed C2 and 250 mg ADVASEP-7 70034 89139-426 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SCAS 100 mg Background fluorescence quencher 70037 89139-432 Each (100mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C1 5 X 1 mg Ex/Em ~480/600 nm, m=0, n=1 70043 89139-444 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C1 1 mg Ex/Em ~480/600 nm, m=0, n=1 70042 89139-442 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C2 (FM2-10) 5 X 1 mg Ex/Em ~480/600 nm, m=1, n=1 70045 89139-448 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C2 (FM2-10) 1 mg Ex/Em ~480/600 nm, m=1, n=1 70044 89139-446 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C3 5 X 1 mg Ex/Em ~480/600 nm, m=2, n=1 70026 89139-410 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C3 1 mg Ex/Em ~480/600 nm, m=2, n=1 70023 89139-404 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C4 (FM1-43) 5 X 1 mg Ex/Em ~480/600 nm, m=3, n=1 70022 89139-402 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoGreen™ C4 (FM1-43) 1 mg Ex/Em ~480/600 nm, m=3, n=1 70020 89139-398 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoRed™ C1 1 mg Ex/Em ~510/750 nm, m=0, n=3 70040 89139-438 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoRed™ C1 5 X 1 mg Ex/Em ~510/750 nm, m=0, n=3 70041 89139-440 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoRed™ C2 (FM4-64) 1 mg Ex/Em ~510/750 nm, m=1, n=3 70021 89139-400 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoRed™ C2 (FM4-64) 5 X 1 mg Ex/Em ~510/750 nm, m=1, n=3 70027 89139-412 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoRed™ C2M (FM5-95) 1 mg Ex/Em ~510/750 nm, m=1, n=3 70028 89139-414 Each (5mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

SynaptoRed™ C2M (FM5-95) 5 X 1 mg Ex/Em ~510/750 nm, m=1, n=3 70019 89139-396 Each (1mg) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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  • State issued document with your organization's Federal Tax ID Number
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  • City or County issued Business License
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* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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