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Cell Viability and Proliferation Assays, Biotium

Supplier: Biotium
30002 30026 30020-2 30020-1 30025-1 30025-2 30050 30006 30007
Biotium offers cell proliferation assays to quantitate living cells by absorbance, fluorescence, or luminescence

Calcein-AM and ViaFluor™ CFSE are initially colorless compounds that are hydrolyzed by cellular esterases to label the cytoplasm with green fluorescence. Calcein-AM and ViaFluor™ CFSE can be used to quantitate viable cells by fluorescence microplate reader. ViaFluor™ CFSE stably labels cells and also can be used to track cell divisions over multiple generations by flow cytometry analysis in the FITC channel. The Cytotoxicity and Viability Assay pairs calcein-AM with the red fluorescent vital dye ethidium homodimer III for quantitation of live and dead cells.

The ATP-Glo cell viability assay uses Firefly luciferase lumenescence to measure cellular ATP levels. ATP-Glo is highly sensitive, and can detect as little as one cell or 0.01 picomole of ATP, with signal linearity for ATP detection within 6 orders of magnitude. ATP-Glo is a flash-type luminescence assay (signal is stable for ~ 1 minute), for use with a single read luminometer or luminescence plate reader with sample injector.

MTT and XTT are reduced by viable cells to form colored formazin salts, which can be measured by absorbance. MTT requires cell lysis prior to measurement, while XTT does not. Resazurin is reduced by viable cells from a dark blue colored compound to a red fluorescent compound, which can be measured by fluroescence or absorbance.

Ordering information: Kit size based on 96-well assay. CFDA-SE assay number varies by application.




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Description Features Kit Size Supplier No. VWR Catalog Number Unit Price Quantity
ATP-Glo Bioluminometric Cell Viability Assay Luminescent viable cell quantitation 1000 Assays 30020-2 89138-940 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

ATP-Glo Bioluminometric Cell Viability Assay Luminescent viable cell quantitation 200 Assays 30020-1 89138-938 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Calcein-AM Cell Viability Assay Kit Quantitate viable cells with green fluorescence 1000 Assays 30026 89138-954 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

MTT Cell Viability Assay Kit Colorimetric viable cell quantitation, requires cell lysis 1000 Assays 30006 89138-906 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Resazurin Cell Viability Assay Kit Red fluorescent or colorimetric viable cell quantitation 2500 Assays 30025-1 89138-950 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Resazurin Cell Viability Assay Kit Red fluorescent or colorimetric viable cell quantitation 10000 Assays 30025-2 89138-952 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

Viability/Cytotoxicity Assay Kit for Animal Live and Dead Cells Fluorescently stain viable cells green and necrotic cells red 300 Assays 30002 89138-886 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

ViaFluor™ CFSE Cell Proliferation Kit Track cell divisions by flow cytometry with green fluorescence 1 Kit 30050 89138-974 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

XTT Cell Viability Assay Kit Colorimetric viable cell quanitation, does not require cell lysis 1000 Assays 30007 89138-908 Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
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  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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