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Anti-NFKB1 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemical
NFkB gel shift assays are assembled in 20µl reactions containing 0.28 pmoles NFkB oligo in 10mM Tris (pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10% glycerol. Some procedures specify the addition of 0.05% NP-40.

Recommended Dilutions: ELISA: 1:5000 - 1:25000; GelShift: 0.5 µl - 1.0 µl; Immunofluorescence: 1:200; Western Blot: 1:2000; Immunochemistry: 1:200;

Type: Primary
Antigen: NFKB1
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Isotype: IgG
Reactivity: Human

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Size Supplier No. VWR Catalog Number Unit Price Quantity
100 µg 600-401-271 RL600-401-271 Each (100µG) Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Requires a maximum 2 day delivery.

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Antigen Symbol NFKB1
Antigen Name Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1
Reactivity Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody Type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ImmunoChemistry Yes
ImmunoFluorescence Yes
Gel Shift Yes
Antigen Synonyms NF-kappa-B, EBP-1, NFKB-p50, NF-kappaB, KBF1, p105, NF-kB1, NFKB-p105, p50, NFkappaB
Gene ID 5970
Concentration 1.0 mg/ml by UV absorbance at 280 nm
Format Antibody fraction
Cross Adsorption No
Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkB p65 peptide corresponding to the NLS of the human Protein conjugated to KLH using maleimide.  A residue of cysteine was added to the amino terminal end to
Purification affinity-purified
Form Liquid (sterile filtered)
Storage Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide,
Storage Temperature -20°C
Shipping Temperature Dry Ice
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