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Anti-GFP Mouse Monoclonal Antibody (AP (Alkaline Phosphatase)) [clone: 9F9.F9]

Supplier: Rockland Immunochemical
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant Proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen.

Recommended Dilutions: ELISA: 1:2000 - 1:12000; Western Blot: 1:500 - 1:2500; Immunochemistry: 1:200 - 1:1000;
Do not freeze!

Type: Tag
Antigen: GFP
Clonality: Monoclonal
Clone: 9F9.F9
Conjugation: AP (Alkaline Phosphatase)
Epitope: Full length
Host: Mouse
Isotype: IgG1 kappa
Reactivity: Pan



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Description Size Source Supplier No. VWR Catalog Number Unit Price Quantity
GFP, Polyclonal 1 mg Aequorea victoria 600-305-215 RL600-305-215 Each (1mg) Retrieving Restricted
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SPECIFICATIONS

Antigen Symbol GFP
Antigen Name Green Fluorescence Protein
Reactivity All Species
Conjugation AP (Alkaline Phosphatase)
Clonality Monoclonal
Antibody Type Tag
Clone 9F9.F9
Host Mouse
Isotype IgG1 kappa
Western Blot Yes
ELISA Yes
ImmunoChemistry Yes
Antigen Synonyms GFPuv, Green Fluorescent Protein, eGFP
Epitope Full length
Concentration 1.0 mg/ml by UV absorbance at 280 nm
Format Antibody fraction
Cross Adsorption No
Immunogen Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion Protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Purification affinity-purified
Form Liquid (sterile filtered)
Storage Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 0.01% (w/v) Sodium Azide, 10 mg/ml Bovine Serum Albumin. Immunoglobulin and Protease free.
Storage Temperature 4°C
Shipping Temperature Wet Ice
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