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XIT™ DNA for Protein-Free DNA Isolation, G-Biosciences

Supplier: G-Biosciences
XIT™
786-294 786-295 786-350 786-340 786-296 786-290 786-301 786-345 786-346 786-347 786-303 786-348 786-304 786-337 786-297 786-341 786-298 786-342 786-343 786-344 786-349 786-338 786-339
G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences' XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation, and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, Buccal cells, plant tissues, mouse tail, yeast, and FFPE tissue. G-Biosciences' XIT™ DNA kit procedures remove contaminants and enzyme inhibitors, allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences' XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio, between 1.7 and 1.9 (with the exception of the Buccal cells kit with a ratio between 1.8 and 2.0), and are up to 200 kb in size.

G-Biosciences' XIT™ Genomic DNA kits for Animal/Plant/FFPE Tissues, Mouse Tail, and Buccal Cells all use a three main step procedure that includes: rapid and efficient cell lysis then enzymatic protein digestion and complete protein precipitation, followed by DNA precipitation.

G-Biosciences' XIT™ Genomic DNA from Tissue kit is designed for the isolation of genomic DNA from fresh, frozen or methanol/acetone fixed tissues. The kit is supplied with three protocols for the isolation of: 1 - 10 mg fresh or frozen tissue, 50 - 100 mg fresh or frozen tissue or 5 - 10 mg fixed tissue.

G-Biosciences' XIT™ Genomic DNA from Plant Tissue kit is designed for the isolation of genomic DNA from fresh or frozen plant tissues.

G-Biosciences' XIT™ Genomic DNA from FFPE Tissue kit is designed for the isolation of genomic DNA from formalin fixed, paraffin embedded tissue.

G-Biosciences' XIT™ Genomic DNA from Mouse Tail kit is designed for the isolation of genomic DNA from 5 mm sections of mouse tail.
G-Biosciences' XIT™ Genomic DNA from Blood kit is designed for the isolation of genomic DNA from blood, bone marrow and buffy coat. The kit uses four main steps that are red blood cell lysis, rapid & efficient nuclear lysis, complete protein precipitation followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Cells kit is designed for the isolation of genomic DNA from adherent or suspension cultured cells. The kit uses rapid & efficient nuclear lysis and complete protein precipitation, followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Gram Negative Bacteria kit is designed for the isolation of genomic DNA from overnight cultures. XIT™ Genomic DNA from Gram Negative Bacteria kit protocol is designed to use 1 ml overnight culture; however the protocol can be easily adapted for larger tissue sample sizes. The kit uses three main steps that are rapid & efficient bacterial lysis, complete protein precipitation, followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Gram Positive Bacteria kit is designed for the isolation of genomic DNA from overnight cultures. The kit uses three main steps that are rapid & efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation.
G-Biosciences' XIT™ Genomic DNA from Yeast kit is designed for the isolation of genomic DNA from overnight yeast cultures. The kit uses three main steps that are rapid and efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation. XIT™ Genomic DNA from Yeast kit protocol is designed to use 1 ml overnight culture; however, the protocol can be easily adapted for larger tissue sample sizes.
G-Biosciences' XIT™ Mitochondrial DNA kit combines G-Biosciences FOCUS™ Mitochondria technology and XIT™ DNA isolation technology in a single kit. The kit uses subcell buffers to lyse cells and animal tissues and remove cellular proteins and nuceli from a highly enriched, intact and active mitochondrial fraction. The mitochondria are then lyzed with the included lysis buffer. The lysate is treated with G-Biosciences' LongLife™ Proteinase K to degrade the proteins, which are then precipitated and the mitochondrial DNA is isolated with alcohol precipitation. The kit protocol also contains protocols for isolation of mitochondra from soft (brain and liver) and hard (cardiac and skeletal muscle) tissue.

Ordering information: Accessories available.




 

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Description Size Kit Size Sample Type Yield Supplier No. VWR Catalog Number Unit Price Quantity
XIT™ DNA from Blood - - Blood 10-15 µg/mL blood 786-294 95057-628 Each (13ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Blood - - Blood 10-15 µg/mL blood 786-296 95057-632 Each (250ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Blood - - Blood 10-15 µg/mL blood 786-295 95057-630 Each (125ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

XIT™ DNA from Buccal Cells (mouthwash) - 25 Preps Buccal Cells 3-5 µg/swab 786-341 95057-650 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Buccal Cells (mouthwash) - 250 Preps Buccal Cells 3-5 µg/swab 786-342 95057-652 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Buccal Cells (with collection brushes) - 50 Preps Buccal Cells 3-5 µg/swab 786-344 95057-656 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

XIT™ DNA from Buccal Cells (with collection brushes) - 25 Preps Buccal Cells 3-5 µg/swab 786-343 95057-654 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

XIT™ DNA from Cells - - - 5-10 µg/1-2 x 10⁶ cells 786-303 95057-638 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Cells - - - 5-10 µg/1-2 x 10⁶ cells 786-304 95057-640 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from FFPE Tissue - - Tissue 0.5-10 µg/mg tissue 786-290 95057-626 Each (250mg) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Gram negative bacteria - - Bacteria 20-40 µg/mg tissue 786-337 95057-642 Each (25ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Gram negative bacteria - - Bacteria 20-40 µg/mg tissue 786-338 95057-644 Each (250ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Gram positive bacteria - - Bacteria 15-25 µg/mL bacterial culture 786-339 95057-646 Each (25ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Gram positive bacteria - - Bacteria 15-25 µg/mL bacterial culture 786-340 95057-648 Each (250ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Mouse Tail For 125 mm mouse tail - Tail 125 mm mouse tail 786-350 95057-668 Each (125mm) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Plant Tissue - - Plant 1-5 µg/mg tissue 786-298 95057-636 Each (25g) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Plant Tissue - - Plant 1-5 µg/mg tissue 786-297 95057-634 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Tissue - - Tissue 0.5-10 µg/mg tissue 786-346 95057-660 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

XIT™ DNA from Tissue - - Tissue 0.5-10 µg/mg tissue 786-345 95057-658 Each (250mg) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Tissue - - Tissue 0.5-10 µg/mg tissue 786-347 95057-662 Each (10g) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Yeast - - Yeast 1-6 µg/mg tissue 786-348 95057-664 Each (25ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ DNA from Yeast - - Yeast 1-6 µg/mg tissue 786-349 95057-666 Each (250ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
XIT™ Mitochondrial DNA - 100 Preps Mitochondria 20 x 10⁶ mammalian cells/prep 786-301 89167-750 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

View MSDS SDS
restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
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  • City or County issued Business License
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* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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