Ponceau S 0.1% in dil. acetic acid membrane stainSupplier: G-Biosciences
, 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
, 3-Hydroxy-4-((2-sulfo-4-((4-sulfophenyl)diazenyl)phenyl)diazenyl)naphthalene-2,7-disulfonic acid, tetrasodium salt
Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*. Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and it also binds non‐covalently to non‐polar regions in the protein.
Microgram quantities of transferred protein can be detected with Ponceau‐S Stain, which generates reddish pink protein bands with a clear background. Since, Ponceau‐S staining is reversible, it allows further immunological detection. The limit of detection for this stain is 100ng BSA/band transferred to nitrocellulose membranes (Aebersold, R. et al (1987) Proc. Natl. Acad. Sci. USA, 84:6970).
This stain does not produce a deleterious effect on the sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot‐sequencing.
*This stain is not suitable for Nylon membranes, since it binds strongly to the membrane because the nylon is positively charged and the stain is negatively charged, therefore the stain is electrostatically held and does not destain
MW: 760.56 g/mol
Storage Temperature: Ambient
CAS Number: 6226-79-5
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