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2D Electrophoresis Reagents, G-Biosciences

Supplier: G-Biosciences
786-230 PSC-03 PSC-04 PSC-05 786-224 PSC-01 786-226 786-227 PSC-02 786-231 786-232
G-Biosciences' FOCUS™ Protein Alkylation Kit contains an ultra pure, proteomic grade iodoacetamide reagent for blocking thiols by alkylation in order to prevent the reoxidation of proteins

G-Biosciences offers a proteomic grade Iodoacetamide in two different quantities: a 5 gram bulk supply or a OneQuant™ format (single aliquots of iodoacetamide that eliminate the need for weighing, preventing loss of reagent and saving time). For the OneQuant™ Iodoacetamide, add 150μL water to a single tube to generate a 0.5M iodoacetamide solution. To improve resolution and prevent streaking of protein spots on 2D gels, G-Biosciences offers an Agarose Sealing Solution and an Equilibration Buffer for IPG Strips. The Equilibration Buffer eliminates urea-induced protein carbamylation or waste of unused reagents, as fresh, ready-to-use reagents are prepared every time.

G-Biosciences' Proteomic Grade Water removes protein and dust contamination to improve the quality and reproducibility of 2D electrophoresis and mass spectrometry results. Offered in sets of 100 are certified protein- and dust-free Proteomic Grade Microfuge Tubes suitable for proteomic analysis and research. G-Biosciences also offers Proteomic Protein Controls for preparations specifically prepared for standardizing electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (< 50μS). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic Protein Sample Controls are available for animal cells, E. coli, yeast, plant, or as a control set that contains one 2 mg vial of each.

Their FOCUS™ Protein Reductant is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] protein reductant with improved stability and efficiency compared to DTT. It reduces proteins over a wide range of pH, including lower acidic pH, and it completely reduces highly stable disulfide bonds in less than 5 minutes at room temperature. FOCUS™ Protein Reductant is compatible with the alkylation of thiol groups for 2D analysis, as it does not compete with the alkylation reagent iodoacetamide, unlike DTT and other commonly used reductants. G-Biosciences' FOCUS™ Reduction-Alkylation kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis that improves resolution and prevents streaking of protein spots on 2D gels. The disulfide bonds are reduced with highly reactive and stable TCEP, followed by blocking of the thiols by alkylation with iodoacetamide reagent preparation. These kits are supplied with the proprietary buffers and reagents necessary for efficient reduction of disulfide bonds and efficient alkylation of thiols, while minimizing reoxidation of the competing thiol pairs in protein samples. G-Biosciences also offers FOCUS™ DTT, an ultra pure proteomic grade DTT, supplied in 5 vials containing dry powder to maintain the activity of the reducing agent. Add 1mL aliquot of water to give a 2M concentration, which is then added to your buffer solution to maintain protein reduction potential.




 

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Description Size Application Supplier No. VWR Catalog Number Unit Price Quantity
Agarose Sealing Solution 50 mL Seals Agarose 786-226 82021-368 Each (50ml) Retrieving Restricted
Equilibration Buffer for IPG Strips 50 mL IPG-Strip Equilibration 786-224 82021-364 Each (50ml) Retrieving Restricted
FOCUS™ DTT [2M] 1 mL Reducing Agent 786-227 82022-658 Pack of 5 (1ml) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

FOCUS™ Protein Alkylation Kit 100 Preps Alkylating Agent for IEF and 2D Electrophoresis 786-232 82022-664 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

FOCUS™ Protein Reductant 100 Preps Reducing Agent for IEF and 2D Electrophoresis 786-230 82022-660 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

FOCUS™ Protein Reduction-Alkylation Kit 100 Preps Reducing and Alkylating Agents for IEF and 2D Electrophoresis 786-231 82022-662 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Protein Proteomic Control, Animal Cells 2 x 2 mg Proteomic Protein Control PSC-02 82022-048 Pack of 2 (2mg) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Protein Proteomic Control, E. coli 2 x 2 mg Proteomic Protein Control PSC-03 82022-050 Pack of 2 (2mg) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Protein Proteomic Control, Plant 2 x 2 mg Proteomic Protein Control PSC-04 82022-052 Pack of 2 (2mg) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Protein Proteomic Control, Yeast 2 x 2 mg Proteomic Protein Control PSC-01 82022-046 Pack of 2 (2mg) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

Protein Proteomic Control Set 4 x 2 mg Proteomic Protein Control PSC-05 82022-054 Pack of 4 (2mg) Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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ACCESSORIES

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Accessories
Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Proteomic Grade Microfuge Tubes (1.5 mL) 100 tubes 786-300 82021-394 Pack of 100 Retrieving Restricted
Proteomic Grade Water 1 L 786-229 82021-370 Each (1l) Retrieving Restricted
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