5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB), Molecular Biology Grade

Supplier: G-Biosciences
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Synonyms: DTNB5,5'-Disulfanediylbis(2-nitrobenzoic acid)2,2'-Dinitro-5,5'-dithiodibenzoic acidEllman's reagent3,3'-Dithiobis(6-nitrobenzoic acid)5,5'-Disulphanediylbis(2-nitrobenzoic acid)

BC87 BC87
82022-846EA 181.23 USD
CA82022-846 82022-846
5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB), Molecular Biology Grade
5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB)

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.


Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.
Store at 2-8°C and protect from light and humidity.


Applications: Quantification of free sulfhydryl residues

Formula: C₁₄H₈N₂O₈S₂
MW: 396.36 g/mol
Melting Pt: 240…244 °C
Storage Temperature: Refrigerator
MDL Number: MFCD00007140
CAS Number: 69-78-3
EINECS: 200-714-4

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