Ficin

Supplier: G-Biosciences
786-793 786-793
89168-042EA 358.23 USD
CA89168-042 89168-042
Ficin
Enzymes
Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason

Immobilized Ficin is a convenient reagent for producing Fab and F(ab')2 fragments as it avoids the need to remove the ficin enzyme after digestion.

Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1mM or 10mM cysteine, ficin generates F(ab')2 and Fab fragments respectively. The Fab and F(ab')2 fragments can be separated from whole IgG and Fc with either Immobilized Protein A or ion exchange chromatography.

Ordering information: Accessories not included.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR