Coomassie brilliant blue G-250, Ultrapure

Supplier: Thermo Scientific Chemicals

Synonyms: Acid Blue 90 Brillant blue G Brilliant indocyanine G Coomassie Blue G 250 Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]-2'-methylbenzhydrylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt Serva Blue G Xylene Brilliant Cyanine G

43318-09 43318-09 43318-18 43318-04 43318-04 43318-18
AA43318-04EA 24.11 USD
CAAA43318-04 AA43318-04 CAAA43318-18 CAAA43318-09 AA43318-18 AA43318-09
Coomassie brilliant blue G-250, Ultrapure
Coomassie brilliant blue G-250

Protein staining following gel electrophoresis

  • E(m) (595nm, H₂O) >36,300

Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.

Formula: C₄₇H₄₈N₃NaO₇S₂
MW: 854.04 g/mol
Density: >1 g/cm³ (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00078482
CAS Number: 6104-58-1
EINECS: 228-058-4

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