Phenol : chloroform : iso-amyl alcohol (125:24:1) (v/v/v) ≥99.0%, Biotechnology Grade, VWR®

Supplier: VWR
Danger

Synonyms: Carbolic acid, Hydroxybenzene, Phenic acid

E277-400ML
97064-744CS 1268.16 USD
97064-744
Phenol : chloroform : iso-amyl alcohol (125:24:1) (v/v/v) ≥99.0%, Biotechnology Grade, VWR®
Phenol : Chloroform : iso-Amyl alcohol

Phenol Chloroform 5:1 Solution. An optimised mixture that can be used directly to replace the phenol and chloroform recommended in the one-step phenol purification method.


  • Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
  • Liquid phenols offer a safer, easier alternative to crystalline phenol
  • Optimized pH for the Purification of RNA
  • Long term stability > one year


For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

MDL Number: MFCD00133763
CAS Number: 136112-00-0
UN: 2810
ADR: 6.1,III

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Specification Test Results

Abs.@ 330 nm 0.2
Abs.@ 405 nm 0.1
Abs.@ 510 nm 0.1
pH @ 25 °C 4.3 – 4.7
Purity 99.0

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