Phenol ≥99.0% saturated with buffer pH 4.5

Supplier: VWR

Synonyms: Carbolic acidHydroxybenzenePhenylalcohol

97064-716CS 839.18 USD
Phenol ≥99.0% saturated with buffer pH 4.5

For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

  • Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
  • Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
  • Optimized pH for the Purification of RNA
  • Long Term Stability > One Year

Formula: C₆H₅OH
MW: 94.11 g/mol
MDL Number: MFCD00002143
CAS Number: 108-95-2
UN: 2821
ADR: 6.1,II

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Specification Test Results

O.D.@ 330nm = 0.2
O.D.@ 405nm = 0.1
O.D.@ 510nm = 0.1
pH (Buffer) @25°C 4.1 - 4.5
Purity =99%

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