Phenol ≥99.0% saturated with buffer pH 4.5
Supplier: VWR
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Danger
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Synonyms:
Carbolic acid
, Hydroxybenzene
, Phenylalcohol
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.
- Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
- Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
- Optimized pH for the Purification of RNA
- Long Term Stability > One Year
Formula:
C₆H₅OH MW: 94.11 g/mol |
MDL Number:
MFCD00002143 CAS Number: 108-95-2 UN: 2821 ADR: 6.1,II |
Specification Test Results
O.D.@ 330nm |
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O.D.@ 405nm |
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O.D.@ 510nm |
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pH (Buffer) @25°C | 4.1 - 4.5 |
Purity |
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About VWR
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