qScript™ One-Step qRT-PCR Kits

Supplier: Quantabio
qScript™
95058-200 95057-200 95059-050 95059-050 95058-200 95059-200 95057-200 95059-200
101414-180EA 730 USD
101414-180 CA101414-172 101414-172 CA101414-176 101414-176 CA101414-178 101414-178 CA101414-180
qScript™ One-Step qRT-PCR Kits
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits
Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures

  • One-step convenience
  • High efficiency and sensitivity
  • Available with reference dye pre-mixed

Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX™ reference dye. Kits 101414-170 and -172 are for use with platforms not requiring passive reference dye, including Roche LightCycler® 480, Bio-Rad® iQ™, MyiQ™, iQ™5, Opticon®, Chromo4™, Corbett Rotor-Gene®, Eppendorf® Mastercycler®, and Cepheid® SmartCycler®. Kits with ROX™ reference dye are for use with platforms including AB 7000, 7300, 7700, and 7900 and StepOne™. Kits with Low ROX™ reference dye are for use with platforms including AB 7500 and Stratagene® Mx™.

Systems have been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. The proprietary reaction buffer has been specifically formulated to maximize activities of both reverse transcriptase and Taq DNA polymerase while minimizing the potential for primer-dimer and other non-specific PCR artifacts. Highly specific amplification is crucial to successful qRT-PCR as non-specific product(s) can compete for amplification of the target sequence and impair PCR efficiency. A key component of this kit is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation at 95°C, the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase.
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