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Immunoprecipitation Starter Pack, GE Healthcare

Supplier: GE Healthcare - Life Sciences
Sepharose™
17-6002-35
Immunoprecipitation is a highly specific and effective technique for analytical separations of target antigens from crude cell lysates

  • High binding capacity with minimal nonspecific adsorption
  • Low leakage

The proteins are immobilized on Sepharose 4 Fast Flow by the cyanogen bromide method. Protein A and protein G have different binding selectivities depending on the origin of the IgG.

In combination with other techniques such as SDS-PAGE and immunoblotting immunoprecipitation can detect and quantify antigens, determine relative molecular weights, monitor protein turnover and posttranslational modifications, and check for enzyme activity.




 

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Description Supplier No. VWR Catalog Number Unit Price Quantity
Immunoprecipitation Starter Pack 17-6002-35 95017-186 Each Retrieving

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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SPECIFICATIONS

Ligand Native Protein A (nProtein A), Recombinant Protein G Lacking Albumin-binding Region (Protein G)
Ligand Coupling Method Cyanogen Bromide Activation
Ligand Density ~6 mg nProtein A/mL, ~2 mg Protein G/mL
Matrix Highly cross-linked agarose, 4%
pH Range 3 - 9
Binding Capacity >35 mg Human IgG/mL medium for nProtein A, >24 mg Human IgG/mL medium for Protein G
Kit Contents Protein A Sepharose 4 Fast Flow (1 x 2 mL), Protein G Sepharose 4 Fast Flow (1 x 2 mL) and Instructions
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