Thermo Scientific™ Pierce™ Spin Cups and Spin Columns are convenient tools for manipulating small volumes of affinity supports (5 to 500µL) for protein purification.
- Smaller pore sizes
- Cellulose acetate filter
- Collection tube cap fits onto inserted spin cup
Simply add the affinity resin and sample to one of the affinity columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process
Efficient washing of samples means fewer washes are needed to remove contaminating proteins. Efficient elution of samples means more antigen and co-precipitated proteins are recovered. No resin loss means more consistent IP and co-IP results. Decanting supernatant from IP or co-IP pellet is not necessary. Spin protocols drastically reduce the time required for IPs and co-IPs. Low protein binding polypropylene column construction minimizes nonspecific binding.
Applications include affinity purification or affinity chromatography, immunodepletion, immunoprecipitation (IP), and co-immunoprecipitation (co-IP). All columns are made from low protein binding polypropylene for compatibility with protein purification. They fit into standard microcentrifuge tubes for use in most microcentrifuges.
Spin columns allow you to affinity purify more protein in less time.
