MMP-9 Human, Cytiva

RPN2614 RPN2614
95019-114EA 960.99 USD
CA95019-114L 95019-114
MMP-9 Human, Cytiva
  • Suitable for use with serum, plasma (EDTA, heparin, citrate) and cell culture supernatant samples
  • Biotrak ELISAs measure MMP protein levels in human serum, plasma, and cell culture samples
  • Simple, convenient, and rapid with high sensitivity
  • Low background
  • Non-isotopic, same day protocol
  • Ready for use substrate

ELISA assays measure the amount of matrix metalloproteinase (MMP) protein. Many normal physiological and pathological processes such as embryogenesis, morphological growth changes, ovulation and pregnancy, wound healing, atherosclerosis, inflammation, tumor invasion, and metastasis involve breakdown and remodeling of the extracellular matrix. This degradation is due to the family of MMP enzymes. Convenient, plate-based colorimetric format.

The Biotrak MMP-9 ELISA provides a simple,. specific, and precise quantitative determination of MMP-9 in tissue. culture supernatants and homogenates as well as plasma. The assay is based on a two-site ELISA sandwich format using two antibodies directed against different epitopes of MMP-9. During the first incubation step, MMP-9 present in the samples or the standards is bound to a microplate pre-coated with antibody. During the second incubation step, detection antibody conjugated to horseradish peroxidase is added, which forms an immobilized complex. The amount of peroxidase bound to each well is determined by the addition of tetramethylbenzidine (TMB) ‘ready-to-use’ substrate. The reaction is stopped by the addition of an acid solution and the resultant color measured at 450nm in a microplate spectrophotometer. The concentration of proMMP-9 in samples is determined by interpolation from a standard curve. MMP-9 may be measured in the range 1–32ng/mL for tissue culture, and the sensitivity of the assay is 0. 6ng/mL. Each pack contains sufficient material for 96 wells. This permits the construction of one standard curve and measurement of 40 unknowns in duplicate. A separate protocol is included for the measurement of MMP-9 in plasma (assay range 4–128ng/mL). The Biotrak MMP-9 ELISA is suitable for research purposes only.

Specific for free pro-MMP-9 and pro-MMP-9 complexed to TIMP-1. No cross-reactivity with pro-MMP-1, -2, -3, TIMP-1, and -2. Suitable for use with cell culture supernatant and plasma samples. 96-wells.

Ordering information: Kit contents include Microplate: plate contains 12 x 8 well strips coated with anti-MMP-9; Assay buffer: Bottle contains 10mL of phosphate buffer concentrate; Standard: Bottle contains 64 ng lyophilized human pro MMP-9; Peroxidase conjugate: Bottle contains lyophilized anti-MMP-9 horseradish peroxidase; Wash buffer: Bottle contains 12.5mL Phosphate buffer concentrate; TMB substrate: Bottle contains 3, 3’ ,5, 5’-Tetramethylbenzidine (TMB)/hydrogen peroxide.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR