Pierce™ O-GlcNAc Western Blot Detection Kit, Thermo Scientific

24565
CAPI24565
Pierce™ O-GlcNAc Western Blot Detection Kit, Thermo Scientific
Pierce™ O-GlcNAc Western Blot Detection Kit contains a specific antibody for the O-GlcNAc post-translational modification (PTM), as well as detection reagents needed to perform a complete detection experiment.

  • Highly specific monoclonal antibody against O-GlcNAc modification
  • Speed and sensitivity of chemiluminescent detection
  • Specific affinity for the beta-O-linked serine or threonine GlcNAc modification

O-Glycosidically-linked modifications of serine or threonine residues are found in proteins from nearly all eukaryotic cells. Although not precisely known, the function of the [beta]-O-linked N-acetylglucosamine (O-GlcNAc) modification may serve as an alternative to phosphorylation, as a modulator of protein interactions in multimeric complexes, or as a fortification against proteolytic attack. The kit contains a highly specific monoclonal mouse antibody that will only link to the [beta] modification. It does not show any cross-reactivity with the α version, the α/β version, or any other N-linked oligosaccharides. The kit also includes SuperSignal® West dura extended duration substrate to allow for visualization of the modified proteins in less than one minute with a sensitivity to the low-picomole range.

Delivery information: The kit contains sufficient reagents for approximately 10 mini blots:
Part A:
M-PER™ Mammalian Protein Extraction Reagent (25 mL), Dilution Buffer, 10X (2 x 50 mL), BupH™ Phosphate Buffered Saline (17 packs), Surfact-Amps™ 20 (10% Tween™ 20 Detergent) (3 x 10 mL), Goat Anti-Mouse IgM (µ), HRP Conjugate (75 µg), SuperSignal™ West Dura Luminol/Enhancer Solution (50 mL), SuperSignal™ West Dura Stable Peroxide Solution (50 mL)
Part B:
Anti-O-GlcNAc Monoclonal Antibody, MAb CTD 110.6 (100 µL), O-GlcNAc Western Blot Positive Control (O-GlcNAc-modified BSA) (100 ng).
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