3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulfate (CHAPS) ≥98%, white powderSupplier: MP BIOMEDICALS (FKA ICN BIOMED
CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.
- pH: 4.0 - 7.0 (10% aq soln)
- Aggregation Number: 4-14 (0-0.1 M Na+)
- Critical Micelle Concentration (CMC): 6-10 mM
CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.
Soluble in water (100 mg/ml - clear, colorless to slightly yellow solution).
Caution: Research Use Only (RUO).
MW: 614.89 g/mol
Melting Pt: 265 °C (lit.)
Storage Temperature: Ambient
CAS Number: 75621-03-3
Merck Index: 12,02085
Specification Test Results
|FTIR||Conforms to Standard|
|OD400 (5% aq soln)||Report Result|
|OD290 (5% aq soln)||Report Result|