NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 5), New England Biolabs

Supplier: New England Biolabs
NEBNext®
E6448S E6448S E6448L E6448L
76540-500EA 2093 USD
76540-500 CA76540-502 76540-502 CA76540-500
NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 5), New England Biolabs
Nucleic Acid Reagents Next Generation Sequencing Reagents
Designed for use in library prep for DNA, ChIP DNA and RNA (but not Small RNA), the NEBNext Adaptors enable high-efficiency adaptor ligation and high library yields, with minimized adaptor-dimer formation.

  • Highly efficient adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Combine with sets 1, 3, and 4 for 384 barcodes

Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER Enzyme (a combination of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 96 pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal.
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