Quick-cfDNA/cfRNA Serum and Plasma Kit

Supplier: Zymo Research
76334-490EA 1080.93 USD
Quick-cfDNA/cfRNA Serum and Plasma Kit
Assays Cellular Assays
The Quick-cfDNA/cfRNA Serum & Plasma Kit enables simple and efficient preparation of all circulating cell-free DNA and RNA (including protein-bound, exosomal, microRNA, and other small RNA) from serum, plasma, and other biological fluids, without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps.

  • Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids, no phenol/chloroform or precipitation
  • Efficient isolation of cell-free DNA and RNA. Recover up to 515 times more microRNA
  • Ready for Next-generation sequencing, RT-qPCR, nCounter, etc

The kit efficiently separates cell-free DNA and RNA, with option to co-elute. Cell-free nucleic acids scale linearly relative to the sample input volume up to 3 ml. Zymo-Spin™ Technology enables isolation of ultra-pure cell-free nucleic acids suitable for all subsequent analyses, such as RT-qPCR and NGS.

cfDNA and cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.

Typically requires 30 minutes (cfDNA/cfRNA co-elution protocol) to 45 minutes (cfDNA/cfRNA separation protocol) hands-on time to process 10 samples. Sample digestion requires 2 hours.

High quality cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.

Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.

Ordering information: Requires the following: Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.

Caution: Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum for both cfDNA and cfRNA. Kit is optimized for recovery of total cfDNA and cfRNA, including small RNA and microRNA.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR