EnzyChrom™ D-Amino Acid Assay Kit, BioAssay Systems

Supplier: BioAssay Systems
EDAA-100
76423-330EA 571.7 USD
76423-330
EnzyChrom™ D-Amino Acid Assay Kit, BioAssay Systems
Assays Enzyme Assays
  • Fast and sensitive: linear detection range - 0.86 to 500 µm (colorimetric assay) and 0.18 to 50 µm (fluorimetric assay) for 60 minutes reaction
  • Method: OD 570 nm or FL530/585 nm
  • Samples: tissue, milk, and other biological samples

D-amino acids are not as widespread as their enantiomeric counterparts in proteins but they can be found in organisms ranging from bacteria (cell walls and antibiotics) to mammals (central nervous systems). The presence of D-amino acids in food is also of considerable interest. Racemization of L-amino acids during food processing may affect food quality and nutritional value.

BioAssay Systems' D-amino acid assay uses an enzyme-catalyzed oxidation of D-amino acids to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the D-amino acid concentration in the sample.
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