MemBrite™ Fix Cell Surface Staining Kits, Biotium

Supplier: Biotium
30095-T 30099 30102-T 30094-T 30103 30101 30104-T 30101-T 30096 30097 30098 30099-T 30102 30093-T 30097-T 30093 30098-T 30096-T 30092-T 30094 30103-T 30092 30104 30095
76403-920EA 187.01 USD
76403-920 76403-914 76403-924 76403-922 76403-918 76403-928 76403-916 76403-926 76294-150 76294-152 76294-130 76294-140 76294-154 76294-132 76294-142 76294-134 76294-144 76294-136 76294-146 76294-116 76294-138 76294-148 76294-126 76294-128
MemBrite™ Fix Cell Surface Staining Kits, Biotium
Cell Strainers
MemBrite™ Fix cell surface staining kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments.

  • Rapid, uniform live cell surface staining
  • Covalent fluorescent labeling can be fixed and permeabilized for IF staining
  • Stain mammalian cells, yeast, or gram-positive bacteria
  • Choose from 12 bright and photostable dye colors from blue to near-IR
  • MemBrite™ Fix-ST dyes for super-resolution STORM imaging

These stains are novel reactive fluorescent dyes stains that react irreversibly with cell surface proteins, for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. Its staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant®, or CellMask™.

MemBrite™ Fix dyes must be used to stain live cells before fixation, and are designed to be fixed shortly after staining; they also can be used to stain yeast and gram-positive bacteria, but not gram-negative bacteria. The dyes are named for their Abs/Em maxima. MemBrite™ Fix-ST dyes are designed specifically for super-resolution imaging by STORM. Other MemBrite™ Fix dyes have been validated in specialized imaging applications including SIM, STED, STORM, TIRF, and 2-photon microscopy; see the ordering table for details.
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