Shiga toxin-producing Escherichia coli detection kit.
- Thermal cycler: Agilent Mx3005P
- Easy and ready-to-use Kit
- Reliable and highly specific Results
- Exclusivity: No cross-reactions with other strains of non- shiga toxin-producing E. coli (STEC)
- Simultaneously detect and differentiate the major STEC-associated virulence marker genes: stx1, stx2 and eae in the same PCR amplification reaction
- Quantification dynamic range: 4 logs
- Amplification limit: 5 DNA copies per reaction
- Fast: results in less than 24 hours with minimal handling
PCR-STEC detection kit is an ideal tool for a specific, rapid, and reliable amplification and detection of the virulence-associated genes stx1, stx2 and eae by the real-time PCR method (Multiplex PCR). The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a buffer, dNTPs, hot-start DNA polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The probe for the detection of target gene is labelled with the FAM (sxt1), HEX (stx2) and ROX (eae) fluorochrome, whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome. In addition, the kit includes both positive control and negative control. The positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a negative control reaction should be included every time the kit is used. Include DNAready lysis buffer to extract the DNA from the sample prior to PCR detection.
