Acridine orange (chlorid salt) solution 10mg/ml in water nucleus stain (DNA/RNA)
Synonyms:
Acridine orange hydrochloride
, Basic Orange 14
, N,N,N’,N’-Tetramethyl-3,6-acridinediamine hydrochloride
, C.I. 46005
, 3,6-Bis(dimethylamino)acridine hydrochloride
, 3,6-Acridinediamine
, AO
Acridine orange (AO) forms a complex with double-stranded DNA to emit green fluorescence (525 nm). AO also forms a complex with single-stranded DNA or RNA to emit red fluorescence (650 nm). One molecule of AO intercalates with three base pairs of double-stranded DNA and emits green fluorescence with the maximum wavelength at 525 nm (excitation 502 nm). One molecule of AO can also interact with one phosphate group of single-stranded DNA or RNA to form an aggregated, or stacked, structure, that emits red fluorescence with the maximum wavelength at 650 nm (excitation 460 nm). Therefore, AO is utilized for the detection of both double-stranded DNA and single-stranded DNA or RNA. It enables simultaneous determination of DNA and RNA with argon laser excitation or flow cytometry. The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies. Acridine orange has also been used for the detection of microorganisms in cerebrospinal fluid and other clinical specimens. We offer a highly purified form of acridine orange while most of the other commercially available grades of AO are either in zinc chloride complex form or of low purity.
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Formula:
C₁₇H₁₉N₃·HCl·xH₂O MW: 301.81 g/mol (anhy) Storage Temperature: Refrigerator |
MDL Number:
MFCD00012660 CAS Number: 65-61-2 |
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