PMA (Propidium monoazide) DNA/RNA binding dye
Synonyms:
SCHEMBL17399073
, Q16821519
, 3-Amino-5-[3-(methyldiethylaminio)propyl]-6-phenyl-8-azidophenanthridinium
, 3-Amino-8-azido-5-{3-[diethyl(methyl)ammonio]propyl}-6-phenylphenanthridinium
High affinity photoreactive DNA binding dye.
PMA is a high affinity photoreactive DNA binding dye that is weakly fluorescent by itself but becomes highly fluorescent upon binding to nucleic acids. It preferentially binds to dsDNA with high affinity. Upon photolysis, the photoreactive azido group on the dye is converted to a highly reactive nitrene radical, which readily reacts with any hydrocarbon moiety at the binding site to form a stable covalent nitrogen-carbon bond, thus resulting in permanent DNA modification (Figure 1). The dye is cell membrane-impermeable and thus can be used to selectively modify DNA from dead cells with compromised membrane integrity, while leaving DNA from viable cells intact. PMA inhibits PCR amplification of modified DNA templates by a combination of removal of modified DNA during purification and inhibition of template amplification by DNA polymerases (Nocker et al. 2006). Consequently the dye is useful in the selective detection of viable pathogenic cells by quantitative real-time PCR.
Spectral Properties:
λabs = 464 nm (before photolysis);
λabs /λem = ~510/~610 nm (following photolysis and reaction with DNA/RNA)
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