sparQ Fast Library Quantification Kits (For Q), Quantabio
95197-500
95197-050
76323-384EA
105.79
USD
76323-384
76323-386
sparQ Fast Library Quantification Kits (For Q), Quantabio
Accurate qPCR-based library quantification in 40 minutes.
- Faster time to results – 50% shorter run time than traditional cycling protocols
- Accurate, reliable quantification of NGS libraries of various sizes and GC-content
- High amplification efficiency across a wide linear dynamic range
- Stabilized, ready-to-use sparQ Fast Mastermix to reduce pipetting steps
- Superior run to run uniformity ensuring highly precise measurements
- qPCR is the most accurate method for library quantification
sparQ PureMag Beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers; primer-dimers; unincorporated nucleotides; salts; adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing; allowing seamless integration into existing workflows. sparQ Fast library quant kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms. Accurate quantification of the number of amplifiable library molecules prior to loading onto a flow cell is a critical step in the NGS workflow and it ensures optimal cluster generation and cost-effective use of sequencing capacity. The sparQ fast library quant kit uses real-time quantitative PCR (qPCR) to specifically quantify the number of library molecules that possess the appropriate adapter tag at each end.
Accurate library quantification in 40 minutes
This kit is optimized for the Q qPCR instrument which uses a magnetic induction technology to rapidly heat samples coupled with fan forced air for cooling to acquire data more rapidly. The combination of the sparQ fast library quant kit and the Q instrument enables fast cycling, reducing qPCR run time by 50% compared to traditional cycling protocols.
This kit is optimized for the Q qPCR instrument which uses a magnetic induction technology to rapidly heat samples coupled with fan forced air for cooling to acquire data more rapidly. The combination of the sparQ fast library quant kit and the Q instrument enables fast cycling, reducing qPCR run time by 50% compared to traditional cycling protocols.
Complete library quantification solution with unmatched convenience
sparQ Fast Library Quant Kit contains six stabilized, pre-diluted DNA standards, ready-to-use 1.25x mastermix pre-mixed with primer sets containing Illumina® P5 and P7 sequences, and an optimized buffer for diluting NGS library samples. This unique formulation minimizes pipetting steps and ensures precise qPCR results.
sparQ Fast Library Quant Kit contains six stabilized, pre-diluted DNA standards, ready-to-use 1.25x mastermix pre-mixed with primer sets containing Illumina® P5 and P7 sequences, and an optimized buffer for diluting NGS library samples. This unique formulation minimizes pipetting steps and ensures precise qPCR results.
qPCR as the most accurate method for library quantification
Real-time quantitative PCR is the most sensitive and precise method for quantifying adapter-ligated DNA molecules. Other methods based on spectrophotometry, fluorometry, or microfluidic electrophoresis (e.g. nanodrop, Qubit, or Bioanalyzer) are acceptable for estimating the appropriate dilutions to use for library quantification. These methods, however, are prone to variabilities and inaccuracies due to factors such as sensitivity to contaminants or measurement of unsequenceable fragments. The qPCR-based sparQ Fast Library Quant Kit measures only library DNA fragments containing the appropriate adapter sequences on both ends and thus serves as the most accurate method for library quantification, which in turn facilitates optimal loading onto sequencing flow cells.
Real-time quantitative PCR is the most sensitive and precise method for quantifying adapter-ligated DNA molecules. Other methods based on spectrophotometry, fluorometry, or microfluidic electrophoresis (e.g. nanodrop, Qubit, or Bioanalyzer) are acceptable for estimating the appropriate dilutions to use for library quantification. These methods, however, are prone to variabilities and inaccuracies due to factors such as sensitivity to contaminants or measurement of unsequenceable fragments. The qPCR-based sparQ Fast Library Quant Kit measures only library DNA fragments containing the appropriate adapter sequences on both ends and thus serves as the most accurate method for library quantification, which in turn facilitates optimal loading onto sequencing flow cells.
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