Ethidium Homodimer III (EthD-III) 1 mM in DMSO used in nuclear staining

Supplier: Biotium

40051
89139-130EA 433.07 USD
89139-130
Ethidium Homodimer III (EthD-III) 1 mM in DMSO used in nuclear staining
Ethidium Homodimer III (EthD-III)

A red fluorescent dead cell stain for bacteria and mammalian cells


  • λEx/λEm (with DNA) = 522/593 nm* (EthD-III also has a large absorption peak at 279 nm)
  • Cell permeability: Membrane impermeant
  • Colors: Red
  • Store at 4°C and protect from light


Ethidium Homodimer III, also known as EthD-III, is a red fluorescent dead cell stain for bacteria and mammalian cells. It is a cell membrane-impermeant nucleic acid dye that stains only dead cells with damaged cell membranes. Ethidium Homodimer III was developed by Biotium as a superior alternative to Ethidium Homodimer I. The absorption and emission spectra are similar, but EthD-III is 45% brighter.


Ethidium Homodimer III is membrane impermeant and useful for selectively staining dead cells with damaged plasma membranes. It also is a component of several combination viability assay kits for detecting both live and dead cells in the same population, such as our Viability/Cytotoxicity Assay Kit for Animal Cells (Cat. No 30002) and our Viability/Cytotoxicity Assay Kit for Bacteria (Cat. No. 30027).


We also offer Ethidium Homodimer III in solid form (catalog no. 40050).


EthD-III is dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.

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