4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride) 10 mg/mL in water used in nuclear staining

Supplier: Biotium

Synonyms: 2-(4-Amidinophenyl)indole-6-carboxamidine DihydrochlorideDAPI.2HCl2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride

40043
89139-118EA 138.53 USD
89139-118
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride) 10 mg/mL in water used in nuclear staining
4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride)

DAPI (4′,6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions.


  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • λEx/λEm (with DNA) = 358/461 nm
  • Yellow solution
  • Store at 4°C and protect from light


Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H2O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate in powder form (catalog no. 40009). DAPI dilactate is more water soluble than the dihydrochloride form of the dye.


DAPI can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.

Formula: C₁₆H₁₅N₅·2HCl
MW: 350.25 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00012681
CAS Number: 28718-90-3

Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR