REVERT total protein stain is a membrane stain that fluoresces at 700 nm. This does not covalently modify sample proteins and therefore does not affect antibody binding or quantification. Total protein stain works with existing western blot workflow, without needing any additional reagents or special gels. Staining takes less than ten minutes and is compatible with both PVDF and nitrocellulose membranes.
- Quick and compatible
- Accurate normalization
- Reliable analysis
The REVERT stain provides high signal intensity with a broad, linear quantitative range. Stained proteins produce signal that can be detected visibly and by imagers capable of detecting dyes that have excitation and emission maxima near 700 nm. With an imaging system capable of multiplex detection, the total protein stained with total protein stain in the 700 nm channel and image a target protein in the 800 nm channel on the same blot. With a total protein stain, it is able to monitor protein transfer across the entire blot at all molecular weights. This will allow to determine if there are any irregularities.
REVERT total protein stain provides highly efficient protein staining on nitrocellulose or Immobilon®-FL PVDF membranes in under 10 minutes. 10 µg of Jurkat cell lysate was loaded in each lane and separated in 4 to 12% NuPAGE Bis-Tris gels, transferred to nitrocellulose or PVDF membrane, and then imaged with the Odyssey® CLx imaging system in the 700 nm channel.
