Kit can be used to purify antibodies in serum, ascites and cell culture medium from various species.
- Ready-to-use pre-packed spin columns of 0.1 ml bead volume in 20% ethanol
- High binding capacity: Binding of IgG > –30 mg human, mouse or rabbit IgG mg/ml Protein A-Agarose
Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins. BioVision’s Protein A (Cat. No. 6500) is a genetically engineered protein containing five IgG-binding regions of native protein A.
Maximum IgG specificity is achieved by eliminating its cell wall binding region, albumin binding region, and other non-specific regions.
Hi-bindTM protein A-agarose beads (Cat. No. 6520) for purification of IgG contain covalently coupled recombinant protein A to 6% cross-linked agarose beads, the most popular resin for protein purification. BioVision’s protein A mini antibody purification kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 30 mg/ml of IgG.
The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.