Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
- Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells.
- DPBS is used to to irrigate, wash, and dilute mammalian cells.
- Phosphate buffering maintains the pH in the physiological range.
- Calcium and magnesium facilitate cell binding and clumping.
- DPBS without these ions can be used to wash and rinse suspended cells.
Inorganic Salts: Potassium Chloride [KCl]: 200.00 mg/L; Potassium Phosphate Monobasic [KH2PO4]: 200.00 mg/L; Sodium Chloride [NaCl]: 8000.00 mg/L; Sodium Phosphate Dibasic [Na2HPO4]: 1150.00 mg/L
Sterile
Osmolality: 270 - 305 mM/kg
Clear colorless solution