The Quick-RNA™ FFPE kit provides a simple and reliable method for RNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples.
- High performance sample prep technology for high quality total RNA (including small/micro RNAs) from FFPE tissue samples and sections
- DNA-free RNA is ready for use in any downstream application
- DNase I included
- Equipment: Microcentrifuge, vortex, heat block/bath
- Purity: RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization
- Sample Source: Up to 25 mg tissue from paraffin block or up to four tissue sections (≤20 µm thick) with a total surface area ~20 mm²
The unique chemistries of the product have been optimized for maximum recovery of both large and small RNA species. Simply deparaffinize tissues using deparaffinization solution, digest using proteinase K, heat to reverse chemical crosslinks, and then purify. The result is high-quality total RNA (including small RNAs 17 to 200 nt) that is DNA-free and is ready for RT-PCR, hybridization and sequencing.
Caution: Stored at less than or equal to -70ºC.