NIH/3T3 Whole Cell Lysate PDGF Stimulated, Rockland Immunochemicals

Supplier: Rockland Immunochemicals
W10-001-377
75930-436EA 142 USD
75930-436
NIH/3T3 Whole Cell Lysate PDGF Stimulated, Rockland Immunochemicals
Cells
NIH/3T3 Whole Cell Lysate PDGF Stimulated

  • Ready-to-use lysates
  • positive controls
  • treated lysated

Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.  All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool.  If dissociating conditions are desired, add reducing agent prior to heating.  The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.
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