HeLa Whole Cell Lysate Nocodozole Stimulated, Rockland Immunochemicals

Supplier: Rockland Immunochemicals
W09-001-A81
75934-382EA 219.08 USD
75934-382
HeLa Whole Cell Lysate Nocodozole Stimulated, Rockland Immunochemicals
Cells
HeLa Whole Cell Lysate Nocodozole Stimulated

  • Ready-to-use lysates
  • positive controls
  • treated lysated

Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.  All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool.  If dissociating conditions are desired, add reducing agent prior to heating.  The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR