Green Fluorescent Protein (GFP) Chemiluminescent Western Blot Kit
- Chemiluminescent Western Blot Kit for GFP contains all of the necessary reagents
- rapid proven protocol - includes straightforward procedures
- extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing GFP and its variants
- This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
GFP Chemiluminescent Western Blot Kit allows for the detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of Rockland's optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.