SETDB2 control protein-GST fusion
- Control Protein
- PCA Assays
- ImmunoBlot
SETDB2 is a histone H3 methyltransferase that modulates gene expression epigenetically through histone H3 methylation. Methylation of histone H3 at lysine 9 (H3K9) by SETDB2 has emerged as an important player in the formation of heterochromatin, chromatin condensation, and transcriptional repression. SETDB2 is recruited to heterochromatin regions and contributes in vivo to the deposition of trimethyl marks. Depletion of SETDB2 coincides with a loss of CENP proteins and delayed mitosis, suggesting that SETDB2 participates in chromosome condensation and segregation (1). Using positional cloning approach, SETDB2 has been cloned from a quantitative trait locus on chromosome 13q14 that influences immunoglobulin E levels and asthma (2). SETDB2 Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.
SEDTB2 Protein is stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. SETDB2 Protein is suitable for use in Western Blot. Expect a band approximately ~120kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.