E. coli Recombinant Caspase 7

Supplier: MBL International

JM-1087-100 JM-1087-25
101974-640EA 578.75 USD
101974-640 101974-642
E. coli Recombinant Caspase 7
Proteins and Peptides
Caspase-7 (also know as Mch3, ICE-LAP3, CMH-1) is a member of the caspasefamily of cysteine proteases. Similar to other caspases, caspase-7 also exists in cells as an inactive proenzyme.

  • Specific activity of about 25000 units/mg

The lyophilized caspase-7 is stable for 1 year at –70 °C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70 °C. Avoid multiple freeze/thaw cycles as activity might decrease.

During apoptosis procaspase-7 is processed at aspartate residues by selfproteolysis and/or cleavage by another caspase. The processed active form of it consists of large and small subunits which associate to form the active enzyme. Active caspase-7 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and genomic maintenance. The recombinant active human caspase-7 was expressed in E. coli. The active caspase-7 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates AcDEVD-pNA or Ac-DEVD-AFC.

Active caspase-7 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity. For a complete caspase-7 assay protocol, please refer to caspase fluorometric or colorimetric assay kits.
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