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Human Recombinant Caspase (active)

Supplier: MBL International

BV-K232-2

101736-434EA 1359.78 USD

101736-434

Human Recombinant Caspase (active)

Proteins and Peptides

Highly active recombinant human caspases were expressed in E. coli and purified. Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining substrate specificity, and as a positive control in caspase activity assays.

Unit defintion: One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8, LEHD-pNA for caspase-9, and IETD-pNA for caspase-10) per hour at 37 °C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1 % Chaps, 10 mM EDTA, 5 % Glycerol, and 10 mM DTT.

Delivery information: Supplied with Recombinant Caspase-1 (25 units), Recombinant Caspase-2 (25 units), Recombinant Caspase-3 (25 units), Recombinant Caspase-6 (25 units), Recombinant Caspase-7 (25 units), Recombinant Caspase-8 (25 units), Recombinant Caspase-9 (25 units) and Recombinant Caspase-10 (25 units).

Caution: For research use only. Not for use in diagnostic purposes.

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