Human Recombinant Caspase 4 (active) (from E. coli)Supplier: MBL International
101974-252EA 578.75 USD101974-252
Human Recombinant Caspase 4 (active) (from E. coli)
Proteins and Peptides
Caspase-4 (also know as Ich-4, ICErelII, TX) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-4 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-4 is a heterotetramer consisting of two large and two small subunits.
Active recombinant human caspase-4 was expressed in E. coli. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA.
Unit definition: One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: p-nitroanaline) per hour at 37 °C in a reaction solution containing 50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Caution: For research use only. Not for use in diagnostic purposes.