Fluorescent dyes for tracking cell divisions by flow cytometry or tracing live cells by microscopy.
- Non-toxic dyes covalently label cell cytoplasm
- Track cell proliferation by dye dilution in flow cytometry
- Long term imaging of cell morphology or tracking cells in co-culture by microscopy
- Violet ViaFluor® 405 offers excellent performance at lower cost
- Green ViaFluor® 488 SE is much less toxic than CFSE
ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. Labeling is covalent, so it withstands fixation and permeabilization for subsequent immunostaining.
The membrane permeable ViaFluor® SE compound is non-fluorescent until it enters viable cells, where it is hydrolyzed by cytoplasmic esterase enzymes to releases the fluorescent amine-reactive dye. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell, while excess unreacted dye is washed away. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labeling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analyzed immediately after staining. Thus, cell proliferation dyes can be used to track multiple cell divisions of cells grown in culture or injected in vivo after labeling with the ViaFluor® SE dye.
ViaFluor® SE dyes also can be used for imaging cell morphology or identifying cells in co-culture by microscopy. Because the staining is non-toxic and well-retained, it can be used for imaging live cells over time.
Ordering information: Trial size kits include 1 vial of dye and 1 vial of anhydrous DMSO for dissolving the dye. Regular size kits include 10 dye vials and 1 vial of anhydrous DMSO.